Cosmid - Net New!

: Often an antibiotic resistance gene (e.g., ampicillin) to identify successfully transformed bacteria. Multiple Cloning Site (MCS)

The cosmid net represents a perfect marriage of virology and bacteriology. By leveraging the packaging precision of a virus and the easy replication of a plasmid, it allows scientists to capture, store, and analyze the vast complexity of life one large piece at a time. cosmid net

The partially digested DNA is run on a sucrose gradient or pulsed-field gel electrophoresis (PFGE). Fragments that are too small (under 30 kb) or too large (over 50 kb) are discarded. Only the "sweet spot" of 40 kb fragments is cut out of the gel and purified. : Often an antibiotic resistance gene (e

So, what is the

Are you designing a or isolating a specific gene cluster ? What is the target size of your DNA insert? The partially digested DNA is run on a

Building a Cosmid Net involves a specific protocol: